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. 2003 May 28;100(12):7389–7394. doi: 10.1073/pnas.1230987100

Fig. 3.

Fig. 3.

Senescence-induced degradation of AtFruct4 requires VPEγ. (A) Total protein was extracted from WT, vpeγ-1, and vpeγ-2 rosette leaves from in vitro-grown plants at the indicated days after germination and analyzed by Western blot with α-VPEγ and α-AtFruct4 antibodies. (B) Transformation with the VPEγ cDNA complements the defect in AtFruct4 degradation. Total protein extracts from senescing rosette leaves of WT, vpeγ-1, vpeγ-2, and four independent vpeγ lines transformed with the VPEγ cDNA under the 35S promoter were analyzed by Western blot with α-VPEγ and α-AtFruct4 antibodies. Positions of intermediate and mature forms of VPEγ are 43 (A) and 40 (B) kDa, respectively. (C) Total protein was extracted from young (Y) or mature (M) leaves of WT and vpeγ-2 plants grown in soil for 14 and 42 days and analyzed by Western blot with α-AtFruct4 antibodies. (D) Total protein was extracted from roots from in vitro-grown WT (lane 1), vpeγ-2 (lane 2), and vpeγ-2 plants expressing the VPEγ cDNA under the 35S promoter (lane 3) at the indicated days after germination and was analyzed by Western blot with α-AtFruct4 antibodies.