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TTX-induced inhibition of myelination is not reversed by PSA removal. TTX (10−6 M) was added for 4 days (10–14 DIV) to the culture medium, and myelination was quantified at 22 DIV. Myelination was decreased by 74.3% in TTX-treated culture. This TTX-induced inhibition of myelination was not prevented by removal of PSA sites by endo-N (0.8 units/ml) added simultaneously in the culture medium between 10 and 18 DIV.
