Figure 6.

Inactive C-terminal mutants cannot functionally complement an inactive RT domain mutant to reconstitute telomerase activity. GST–hTERT D868N and C-terminal mutants of hTERT were co-synthesized in RRL in the presence of hTR and [³⁵S]methionine. Telomerase activity was measured by TRAP assay (top panel). The Δ150–159 mutant (lane 2) is an inactive N-terminal hTERT mutant that functionally complements GST–hTERT D868N to reconstitute telomerase activity (17). The W547A mutant is an inactive N-terminal hTERT mutant (lane 3) that does not functionally complement GST–hTERT D868N to reconstitute telomerase activity (17). GST–hTERT D868N and hTERT mutants were detected by SDS–PAGE/autoradiography (bottom panel).