Table 1. Type II processivity of C-terminal hTERT mutants.
| Mutant | Average repeat addition processivity | Repeat addition processivity relative to wild type |
|---|---|---|
| hTERT | 29.85 ± 7.67 | 100 |
| Δ936–945 | 33.22 ± 8.24 | 111 |
| Δ963–972 | 14.05 ± 4.32* | 47 |
| Δ993–1002 | 25.19 ± 9.99 | 84 |
| Δ1020–1029 | n/a | n/a |
| Δ1047–1056 | 6.86 | 23 |
| Δ1077–1086 | 19.60 ± 9.12 | 66 |
| Δ1108–1117 | 12.81 ± 3.93* | 43 |
| Δ1123–1132 | 26.71 ± 12.54 | 89 |
| L974A | 23.41 ± 21.28 | 78 |
| L978A | 23.60 ± 8.96 | 83 |
| L980A | 21.65 ± 20.74 | 79 |
| T1030A | 25.12 ± 7.56 | 84 |
| S1037A | 23.81 ± 10.19 | 79 |
| S1041A | 29.94 ± 10.11 | 100 |
Processivity values were calculated as described in Materials and Methods. Type II processivity-defective mutants are indicated by bold typeface. Mutants with average processivity values that are significantly different compared with wild-type values (P < 0.05) are indicated by *. n/a indicates second-repeat products whose signal is too weak for quantification of type II processivity. Second-repeat products for mutant Δ1047–1056 were quantifiable only once; thus an average processivity value was not calculated, nor could statistical significance be calculated, despite the greatly reduced processivity.