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. 2003 Jul 15;31(14):4041–4050. doi: 10.1093/nar/gkg442

Figure 5.

Figure 5

Mutational analysis of the Xrcc3–Rad51C interaction by the yeast two-hybrid system. Yeast strains grown on SD plates with histidine (top row) and without histidine (middle row) are shown. The β-galactosidase activities, which were induced by the Xrcc3–Rad51C interaction, were detected by the X-Gal filter assay, and the results are shown in the bottom row. Lane 1 indicates a negative control with an empty vector instead of the Xrcc3 vector and lane 2 indicates a positive control with the wild-type Xrcc3 protein. Lanes 3–8 indicate experiments with the Xrcc3 point mutants, F30A, Y139A, F180A, F219A, F223A and F249A, respectively.