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. 2003 Jul 15;31(14):4256–4263. doi: 10.1093/nar/gkg443

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Study of reverse transcription inhibition by OXNs. (a) Oligo nucleotide sequences. (b) Thermal denaturation–renaturation study of triplexes formed by the DNA target TDIII with two separate OXNs (Xp-pim and Xap-pim) and the clamp X-pim in a buffer containing 50 mM Tris–acetate, 100 mM Na acetate, 10 mM Mg acetate, pH 7.0. (c) PAGE analysis of the pim-1 mRNA reverse transcription products in the absence (lane 2) and presence of OXNs (lanes 3–8). (d) Calculated efficiency of pim-1 cDNA synthesis in the absence and presence of OXNs.

Study of reverse transcription inhibition by OXNs. (a) Oligo nucleotide sequences. (b) Thermal denaturation–renaturation study of triplexes formed by the DNA target TDIII with two separate OXNs (Xp-pim and Xap-pim) and the clamp X-pim in a buffer containing 50 mM Tris–acetate, 100 mM Na acetate, 10 mM Mg acetate, pH 7.0. (c) PAGE analysis of the pim-1 mRNA reverse transcription products in the absence (lane 2) and presence of OXNs (lanes 3–8). (d) Calculated efficiency of pim-1 cDNA synthesis in the absence and presence of OXNs.