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. 2003 Jul 15;31(14):4256–4263. doi: 10.1093/nar/gkg443

Table 1. Thermal stability of oligonucleotide complexes (50 mM sodium cacodylate, 100 mM NaCl, 10 mM MgCl2).

Oligonucleotide system Complex composition Tm (°C) (±0.5°C)
    pH 7.0 pH 5.0
System I TDI-D 48.2 49.0a
  X-TDI-X 47.1 71.0
  TRI-D 58.7  
  X-TRI-X 40.7 71.1
System II TDII-Dap 44.6  
  Xp-TDII-Xap 38.4 63.8
  TRII-Dap 49.2  
  Xp-TRII-Xap 36.0 62

aThe oligonucleotide ratio at pH 5 was D:TDI = 2:1.