Table 2. The influence of monovalent ions on the thermal stability of the triplexes X-TDI-X and X-TRI-X in a buffer containing 50 mM Tris–HCl, pH 7.0, 10 mM MgCl2 and 100 mM NaCl or KCl.
Complex | Tm (°C) (±0.5°C) in the presence of ions | |
---|---|---|
Na+ | K+ | |
X-TDI-X | 59.2 | 56.3 |
X-TRI-X | 54.5 | 47.7 |