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. 2003 Jul 15;31(14):4051–4058. doi: 10.1093/nar/gkg444

Figure 1.

Figure 1

Bioinformatics strategy. All 48 human NR cDNAs were compared against the Fugu proteome database using BLASTX. In a complementary approach, hidden Markov models for the DBD and the LBD were used in a HMMer search. Combining the BLAST results and HMM results, 85 putative Fugu NRs were identified. These hits were reduced to 74 putative orthologs after removing redundant peptide sequences with nrdb95.pl (see Materials and Methods). Partial sequences that represented different sections of the same receptor mRNA sequence were identified by cloning and sequence analysis. The joining of these partial sequences reduced the number of receptor sequences to 69. Finally, one pair of sequences was determined to represent splice variants, reducing the final number of NR sequences to 68.