Figure 3.
Presence of T-channel α1 subunits in fusing myoblasts and myotubes. (A) PCR products of expected sizes for the three α1 subunits were obtained from human fusion-competent myoblasts (FC) and myotubes (MT). Sequencing revealed 100% identity with sequences of human Ca2+ channel subunits. (B) Whole-cell T-type Ca2+ conductances recorded in small myotubes 48 h after cytoplasmic injection of antisense (AS) or scrambled (SCR) oligonucleotides. Between 10 and 13 cells were recorded in each condition.