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. 2000 Jun 20;97(13):7627–7632. doi: 10.1073/pnas.97.13.7627

Figure 4.

Figure 4

Ni2+ and amiloride, but not nifedipine, inhibit myoblast fusion. (A and B) Myoblast fusion is inhibited by Ni2+ (A) and amiloride (B), and the effect is reversible. Fusion was induced with differentiation medium and was assessed three times within 3 days in culture. The fusion index is defined as the number of nuclei in myotubes divided by the total number of nuclei counted (16). Fusion in control conditions is represented by squares. In sister cultures, the two compounds were added to the differentiation medium either during the entire experiment (triangles) or for a period of 24–36 h (circles). Error bars are omitted because they are smaller than symbols. (C) Myoblast fusion is not affected by nifedipine. Squares represent the fusion rate in control conditions and triangles in the presence of 10 μM nifedipine. Photographs represent hematoxylin-stained cultures after 3 days in differentiation medium without (CTRL) and with Ni2+, amiloride, or nifedipine. (Bars = 40 μm.)

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