Skip to main content
. 2003 Jul 15;31(14):4024–4030. doi: 10.1093/nar/gkg447

Figure 5.

Figure 5

Figure 5

Tight binding of PolB1 to ssDNA oligonucleotides containing deaminated bases. BIAcore sensograms showing the association and dissociation kinetics for binding of S.solfataricus DNA polymerases to immobilized oligonucleotides. (A) Demonstration of tight binding of PolB1 to oligonucleotides containing deaminated bases (either single uracil or single hypoxanthine as shown) compared to control oligonucleotide without lesions. (B) Comparison of PolY1 and PolB1 binding to control oligonucleotide. Binding to substrates was tested by injecting the appropriate protein independently five times at concentrations of 10, 50, 100, 250 and 500 nM.