Table 1.
Marker | Size, bp | Polymorphism* | Primers | |
---|---|---|---|---|
MYH19L | 175 | SspI: Col=175 | 5′-CTATATATTTTATACAAGTTTAAAATAAATA-3′ | |
Ler=145, 30 | 5′-CTACCATGTGATGACATG-3′ | |||
MYH19VR | 880 | XhoI: Col=610, 270 | 5′-CGCGTGGGTACTCATCTAGTG-3′ | |
Ler=880 | 5′-CGGATTAGCAGCTAGGACAG-3′ | |||
MSN9L | 190 | AflIII: Col=190 | 5′-GACAAGGCTTATTGTTTACGT-3′ | |
Ler=170, 20 | 5′-GAATGTATTATGCGTAGTGAC-3′ | |||
MSN9R | 200 | SpeI: Col=200 | 5′-TGGCAGTAATTTAATGATAAACTA-3′ | |
Ler=180, 20 | 5′-CGTATTGAGCCTACATAACAA-3′ | |||
MPO12VL | 180 | Fnu4HI: Col=160, 20 | 5′-AATTAATTTGAGTTTGTTTGGCAG-3′ | |
Ler=180 | 5′-TATGTTTCAAACAATAGTTTAGG-3′ | |||
MPO12.2 | 220 | HinfI: Col=220 | 5′-GTCTTGTAGACTCTCCAAGACT-3′ | |
Ler=200, 20 | 5′-CTCTTTCTATTATGTTCACCTCT-3′ | |||
MPO12M | 200 | HinfI: Col=200 | 5′-GCTCGAGATAAATATTTGAGACT-3′ | |
Ler=180, 20 | 5′-AGGGATTCTTACTCTTTGTAGG-3′ | |||
MPO12R | 305 | Tth111I: Col=285, 20 | 5′-GTTTCTGATCTGTGGTGACGGA-3′ | |
Ler=305 | 5′-GGCTAATTCTCTCCGGCAC-3′ |
Indicated is the restriction enzyme used and the sizes of the digestion products for Col (Columbia) and Ler.