FIG. 3.

BCG PGN, like BCG CWS, induces TNF-α production by mouse macrophages and DCs through TLR2, TLR4, and MyD88. (A) Thioglycolate-elicited peritoneal macrophages from wild-type mice, TLR2^−/− mice, TLR4^−/− mice, and TLR2^−/− TLR4^−/− double-knockout mice were cultured with BCG CWS (15 μg/ml) or BCG PGN (5 μg/ml) for 24 h. The TNF-α secreted into the culture supernatants was measured by ELISA. The experiment was repeated twice with similar results, and the results of a representative experiment are shown. Mφ, macrophages. (B) Bone marrow-derived DCs were prepared from wild-type mice and MyD88^−/− mice as described in Materials and Methods (15). Cells were cultured with BCG CWS (15 μg/ml) or BCG PGN (5 μg/ml) for 24 h. The concentrations of TNF-α in the culture supernatants were measured by ELISA.