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PMN apoptosis after stimulation with BCG or GM-CSF. PMN were cultured for 24 h in the presence of increasing concentrations of BCG or with GM-CSF. A total of ≥200 cells on three different Giemsa-stained cytocentrifuge slides were assessed for changes in nuclear morphology. The figure bars show the combined result of six independent experiments with the respective percentages of viable versus apoptotic cells given as mean ± SD (error bars) using morphological criteria.
