TABLE 2.
Supernatants of BCG-stimulated PMN induce an upregulation of function-associated molecules on allogeneic PMNa
| Stimulus | Δmean (% increase)
|
|||
|---|---|---|---|---|
| CD11b | CD16 | CD18 | CD32 | |
| Supernatant of unstimulated PMN | 152.8 | 122.7 | 31.1 | 82.1 |
| Supernatant of PMN + 105 CFU of BCG/ml | 158.5 (3.8) | 127.2 (3.7) | 49.9 (60.5) | 84.1 (2.4) |
| Supernatant of PMN + 106 CFU of BCG/ml | 282.1 (84.6) | 155.6 (26.8) | 84.9 (173) | 105.8 (28.9) |
a
PMN were stimulated with increasing concentrations of viable BCG for 24 h. Supernatants were harvested and further processed as described in Materials and Methods. Allogeneic PMN were incubated in these supernatants for 2 h, harvested, and analyzed by flow cytometry. Mean fluorescent intensities from isotype controls were subtracted from the mean of the respective specific stainings and are listed as Δmean. The percentages of change in surface molecule expression are depicted in parentheses. The table shows the representative results of one of three independent experiments.