TABLE 3.
PMN gene expression profile before and after stimulation with BCGa
| Gene | Level of expression
|
Mode of regulation | ||
|---|---|---|---|---|
| Unstimulated | 1.5 h | 3.5 h | ||
| TNFR2 | ++++ | ++++ | ++++ | |
| MIF | ++++ | ++++ | ++++ | |
| β-Actin | ++++ | ++++ | ++++ | |
| MPIF-1 | ++++ | ++++ | +++ | |
| Ribosomal protein L13A | +++ | +++ | +++ | |
| IL-1R2 | +++ | +++ | +++ | |
| TARC | +++ | −/+++ | +++ | |
| SCYB13 | +++ | +++ | +++ | |
| MDC | +++ | +/+++ | +++ | |
| GAPDH | +++ | −/+++ | −/+++ | |
| TECK | +++ | −/+++ | +++ | |
| TGF-β3 | +++ | +/+++ | +++ | |
| SCYA19 | +++ | −/+++ | −/+++ | |
| Peptidylpropyl isomerase | +++ | +++ | ++ | |
| IL-21 | +++ | −/+++ | +++ | |
| IL-16 | +++ | ++ | +++ | |
| IL-17R | ++ | ++ | + | |
| CXCR4 | ++/++++ | ++/++++ | ++/++++ | |
| IL-1β | ++ | ++/++++ | +++ | Up |
| IL-2Rγ | ++ | +++ | +++ | Up |
| IL-11 | ++ | −/++ | ++ | |
| IL-1R1 | ++ | −/++ | +/+++ | |
| IL-10Rα | ++ | ++ | +++ | Up |
| IL-15 | ++ | −/++ | −/++ | Down |
| Eotaxin | ++ | −/++ | −/++ | |
| MIP-1β | ++ | +/++++ | +/++++ | Up |
| IL-10Rβ | ++ | −/++ | + | |
| IL-13Rα1 | +/+++ | +/+++ | −/++ | Down |
| IL-6R | +/+++ | ++ | +++ | Up |
| IL-25 | + | −/++ | + | |
| IL-5Rα | + | −/++ | − | |
| IL-9 | + | − | −/++ | Down |
| GCP-2 | −/++ | −/++ | −/++ | |
| CCXCR1 | −/++ | − | − | |
| IL-12α | −/++ | − | − | Off |
| IL-11Rα | −/++ | − | − | |
| IL-17 | −/++ | − | − | |
| SDF-2 | −/+++ | −/++ | − | |
| TGF-β1 | −/+++ | −/++ | −/++ | |
| MIP-1α | −/++ | −/+++ | −/++++ | De novo |
| I-309/SCYA1 | −/+++ | −/+++ | −/+++ | |
| MIP-1δ | −/++ | −/++ | −/++ | |
| CCR1 | −/++ | − | − | |
| CCR3 | −/++ | −/++ | −/++ | |
| IL-10 | − | − | − | |
| IL-12Rβ1 | − | − | − | |
| IL-15Rα | − | − | − | |
| CCR7 | − | − | − | |
| HCC-1/SCYA14 | − | − | − | |
| IL-18R1 | − | − | − | |
| CCR5 | − | − | − | |
| IL-2Rβ | − | −/++ | − | |
| MIP-3α | − | − | −/++ | |
| CCR2 | − | − | − | |
| IL-13 | − | − | − | |
| LTA | − | − | − | |
| RANTES | − | − | −/++ | |
| IL-1α | − | + | +/+++ | De novo |
| SCYC2 | − | −/++ | −/++ | |
| CCR4 | − | − | − | |
| IL-18 | − | − | − | |
| MCP-4 | − | − | − | |
| IL-13Rα2 | − | − | − | |
| IL-2Rα | − | − | − | |
| IL-12Rβ2 | − | − | − | |
| IL-6 | − | − | − | /PICK> |
| CCR6 | − | − | − | |
| blank | − | − | − | |
| Burkitt lymphoma R1 | − | − | − | |
| CCR8 | − | − | − | |
| CCR9 | − | − | − | |
| CX3CR1 | − | − | − | |
| IFN-γ | − | − | − | |
| IL-12β | − | − | − | |
| IL-2 | − | − | − | |
| IL-20 | − | − | − | |
| IL-4 | − | − | − | |
| IL-5 | − | − | − | |
| IL-6 signal transd./gp130 | − | − | − | |
| IL-9R | − | − | − | |
| Leptin | − | − | − | |
| Lymphotoxin β/LTB | − | − | − | |
| LTBR | − | − | − | |
| puc18 | − | − | − | |
| HCC-4/ SCYA16 | − | − | − | |
| PARC | − | − | −/+++ | |
| MCP-1 | − | − | − | |
| MIP-2 | − | − | − | |
| MPIF-2 | − | − | − | |
| MCP-3 | − | − | − | |
| MCP-2 | − | − | − | |
| P10/IP10 | − | − | − | |
| I-TAC/IP9 | − | − | − | |
| ENA-78/ SCYB5 | − | − | − | |
| Lymphotactin | − | − | − | |
| Fractalkine | − | − | − | |
| SCYE1 | − | − | − | |
| SDF-1 | − | − | − | |
| TGF-α | − | − | − | |
| TGF-β2 | − | − | − | |
| TNFα | − | − | − | |
| TNFR1 | − | − | − | |
PMN were stimulated with 106 CFU of viable BCG/ml for 1.5 or 3.5 h. Total RNA was prepared, and messages were specifically identified with radiolabeled cDNA probes as described in Materials and Methods. Resulting cDNAs were hybridized on SuperArray nylon membrane microarrays and analyzed with a PhosphorImager. The table shows the combined result of three independent experiments. Using Phoretix Analyzing software (Version 3.01), total values were converted and grouped into five different expression levels: −, no expression; +, low expression; ++, moderate expression; +++, high expression; ++++, very high expression. Where more than one result is given, results separated by the slash show that expression was variable between experiments. Equal expression levels of + or higher for unstimulated as well as stimulated PMN denote constitutive gene expression. A >2-fold change in total expression level (either up or down) was considered relevant and used as a cutoff.