TABLE 4.

BCG-induced changes in PMN gene expression^a

Mode of regulation	Regulated gene(s) (kinetics of regulation)
Up	IL-10Rα (4-fold after 3.5 h), IL-1β (5-fold after 1.5 h and 13-fold after 3.5 h), IL-2Rγ (2-fold after 3.5 h), IL-6R (3-fold after 1.5 h and 6.4-fold after 3.5 h), MIP-1β (12-fold after 3.5 h)
De novo	IL-1α (after 1.5 h and 3.5 h), MIP-1α (after 3.5 h)
Down	IL-13Rα1 (0.2-fold after 1.5 h), IL-15 (0.4-fold after 1.5 h), IL-9 (0.2-fold after 1.5 h)
Off	IL-12α (after 1.5 h), IL-5R (after 1.5 h)

^aPMN were stimulated with 10⁶ CFU of viable BCG/ml for 1.5 or 3.5 h. Total RNA was prepared, and messages were specifically identified with radiolabelled cDNA probes as described in Materials and Methods. Resulting cDNAs were hybridized on SuperArray nylon membrane microarrays and analyzed with a PhosphorImager. The table shows the combined results of three independent experiments. Using Phoretix Analyzing software (Version 3.01), total values were converted and grouped into four different modes of regulation (up, de novo synthesis, down, or switched off). A >2-fold change in total expression level (either up or down) was considered relevant and used as a cutoff.