FIG. 2.

Evaluation of antigenic epitopes. (A) Alignment of recovered epitopes to individual ORFs from S. aureus. SA2511 (coding for fibronectin-binding protein, FnbpA), SA0835, SA0021, and SA1777 are shown as examples. For fnbpA, PCR products amplified from the input library are shown. The sense primer binds to the indicated regions of fnbpA, whereas the antisense oligonucleotide hybridizes to the constant region of the β-lactamase spacer. (B) Enrichment of frequently found S. aureus library fragments during the selection process. Enrichment was determined by semiquantitative PCR with clone-specific primers. The sense primer hybridizes to the indicated S. aureus library fragment (compare Table 1). The antisense primer binds to the constant β-lactamase spacer. Each lane shows the PCR product of a different template: inp., DNA of input library; 1 to 5, cDNA of the first through fifth round, respectively, of ribosome display screened with normal high titer serum. Marker (M) length is shown on the left.