TABLE 1.
Immunoreactive S. aureus genes identified in this studya
| TIGR locus | Gene name or function | No. of clonesb | Reactivityc
|
IPd | % Conservatione | TM/Sf | Region(s)g (amino acid) | |
|---|---|---|---|---|---|---|---|---|
| P sera | N sera | |||||||
| SA0006 | DNA gyrase, A subunit (gyrA) | 5 | 9/14 | 15/19 | ND | 81.08 | ND | 89-130 |
| SA0021 | yycH protein (yycH) | 53 | ND | 100.00 | ND | 8-24, 394-494 | ||
| SA0050 | Methicillin-resistant surface protein (pls) | 6 | + | 54.05 | ND | 1191-1255, 1389-1496 | ||
| SA0103 | Drug transporter, putative | 8 | 14/14 | 18/19 | ND | 100.00 | ND | 84-100 |
| SA0195 | Maltose ABC transporter, permease protein | 100 | 14/42 | 4/32 | + | 100.00 | ND | 78-129 |
| SA0200 | Phosphoglycerate transporter family protein | 379 | 42/42 | 26/32 | + | 94.59 | ND | 323-345 |
| SA0317 | Lipase precursor, interruption | 33 | 2/42 | 10/32 | ND | 75.00 | ND | 54-92 |
| SA0334 | Conserved hypothetical protein | 168 | + | 100.00 | TM/S | 1-43 | ||
| SA0754 | Multidrug resistance protein | 3 | 5/5 | ND | 70.27 | ND | 155-175 | |
| SA0835 | Conserved hypothetical protein | 65 | 40/42 | 30/32 | + | 100.00 | TM/S | 6-54 |
| SA0856 | Clumping factor A (clfA) | 6 | 2/5 | ND | 21.62 | ND | 87-98 | |
| SA0946 | Conserved hypothetical protein | 5 | 2/5 | ND | 75.68 | TM/S | 105-122 | |
| SA0950 | Na+/H+ antiporter, MnhF component (mnhF) | 66 | 2/5 | ND | 66.67 | ND | 1-21 | |
| SA1045 | Iron compound ABC transporter | 10 | 5/5 | ND | 100.00 | ND | 1-18 | |
| SA1062 | Bifunctional autolysin (atl) | 19 | 8/14 | 13/18 | ND | 100.00 | ND | 64-81 |
| SA1112 | Conserved hypothetical protein | 349 | 14/14 | 18/18 | ND | ND | −/− | 13-37 |
| SA1138 | Cell wall surface anchor family protein | 4 | ND | ND | ND | 53-73 | ||
| SA1168 | Fibrinogen-binding protein (fib) | 3 | ND | 100.00 | ND | 127-141 | ||
| SA1259 | Conserved hypothetical protein | 13 | 2/5 | ND | ND | TM/S | 196-218 | |
| SA1743 | Amino acid permease | 9 | 14/14 | 17/18 | ND | 94.59 | ND | 127-152 |
| SA1777 | Serine protease HtrA, putative | 73 | 17/42 | 9/32 | ND | 100.00 | ND | 1-30 |
| SA1806 | Cell wall surface anchor family protein | 9 | 7/14 | 16/18 | ND | 100.00 | ND | 508-523 |
| SA1919 | Transcriptional regulator | 3 | 13/14 | 18/18 | ND | 100.00 | ND | 120-135 |
| SA2125 | Peptidase, M20/M25/M40 | 3 | 13/14 | 18/18 | ND | 97.30 | ND | 293-312 |
| SA2347 | Drug resistance transporter, EmrB/QacA subfarr | 5 | 14/14 | 18/18 | ND | 100.00 | ND | 205-224 |
| SA2437 | Bicyclomycin resistance protein (bcr) | 8 | 13/14 | 18/18 | ND | 100.00 | ND | 54-104 |
| SA2505 | Cell wall surface anchor family protein | 5 | 19/42 | 7/32 | + | ND | ND | 829-849 |
| SA2511 | Fibronectin-binding protein A (fnbA) | 8 | 13/14 | 12/18 | ND | 100.00 | ND | 129-196, 876-913 |
| SA2652 | Clumping factor B (clfB) | 17 | 41/42 | 32/32 | ND | 100.00 | ND | 711-751 |
| SA2659 | Zinc metalloproteinase aureolysin | 12 | 4/42 | 1/32 | ND | 97.30 | ND | 90-107 |
Listed is a representative subset of 75 genes for which three or more independent clones were identified as mapping to the same region. The Institute for Genomic Research (TIGR) locus and gene name are according to the CMR database (20).
Number of clones located in the respective gene.
Reactivity in peptide ELISA with patient (P) sera or normal-subject high-titer (N) sera (as described in reference 4). Data are presented as number of serum samples positive for reactivity/total number of serum samples tested.
Immunoprecipitation reaction: +, positive; ND, not determined.
Conservation of respective genes in 36 S. aureus isolates as determined by DNA chip hybridization (6). ND, not determined.
Prediction of transmembrane domain or signal peptide (TM/S) (ND, not determined).
Selected region(s) in relative amino acid position.