FIG. 2.

Morphological damage in CaCo-2 cells treated with 10 μg of CPE per ml. Confluent CaCo-2 cells were pretreated for 2 h at 37°C with HBSS that contained or did not contain the broad-spectrum caspase inhibitor Z-VAD-FMK (50 μM final concentration) or oncosis inhibitor glycine (5 mM final concentration), as indicated. Those cultures were then treated for 30 or 60 min (as indicated) in HBSS with or without 10 μg of CPE per ml, along with the same inhibitor (if any) used during pretreatment. After treatment, photomicrographs were taken of each culture dish.