FIG. 7.

Effects of CPE on mitochondrial membrane depolarization. Confluent CaCo-2 cells that had been pretreated with a specified caspase or oncosis inhibitor were treated for 15 min at 37°C with HBSS containing 1 or 10 μg of CPE per ml. After treatment, the cells were incubated with JC-1 dye and mitochondrial depolarization was demonstrated by using a FACSCalibur flow cytometer (depolarization is indicated in the lower right quadrant). CaCo-2 cells treated with 2 μM staurosporine for 6 h at 37°C served as a positive control for mitochondrial depolarization. Control, CaCo-2 cells treated only with HBSS (no CPE). The data shown are a representative result from one of three experiments.