TABLE 1.
Development of inflammatory infiltrates and tissue injury in the bovine and murine ileal mucosa following inoculation with the serotype Typhimurium wild type, the sipA sopABDE2 mutant, or sterile LB broth
| Loop source | Pathological change | Loop inoculated with | Avga qualitative score of pathological changesb in tissue collected at the following times (h) after inoculation of loops
|
||||
|---|---|---|---|---|---|---|---|
| 0.5 | 1 | 2 | 4 | 8 | |||
| Calves | Tissue-associated PMN infiltration | Wild type | 1.7 | 2.7 | 3.3 | 2.7 | 4.0 |
| sipA sopABDE2 mutant | 1.7 | 1.3 | 2.7 | 1.7 | 2.3 | ||
| LB broth | 2.0 | 2.0 | 2.0 | 1.3 | 1.0 | ||
| Intravascular PMN infiltration | Wild type | 0.7 | 1.7 | 2.3 | 1.3 | 2.3 | |
| sipA sopABDE2 mutant | 1.0 | 0.7 | 2.3 | 2.0 | 1.7 | ||
| LB broth | 1.0 | 0.5 | 1.3 | 1.3 | 1.0 | ||
| Monocyte infiltration | Wild type | 2.0 | 1.7 | 1.7 | 2.3 | 3.0 | |
| sipA sopABDE2 mutant | 1.9 | 1.3 | 2.3 | 1.7 | 2.0 | ||
| LB broth | 1.7 | 2.0 | 1.7 | 1.7 | 1.3 | ||
| Detachment of surface epithelia | Wild type | 0.0 | 1.0 | 1.4 | 1.7 | 3.3 | |
| sipA sopABDE2 mutant | 0.7 | 0.3 | 2.0 | 1.0 | 1.3 | ||
| LB broth | 0.0 | 1.4 | 1.0 | 0.3 | 0.7 | ||
| Mice | Tissue-associated PMN infiltration | Wild type | 0.0 | 0.0 | 0.3 | 0.0 | 0.3 |
| sipA sopABDE2 mutant | 0.0 | 0.3 | 0.7 | 0.7 | 1.3 | ||
| LB broth | 1.0 | 0.0 | 0.0 | 0.0 | 0.3 | ||
| Intravascular PMN infiltration | Wild type | 0.5 | 0.7 | 1.0 | 1.0 | 1.0 | |
| sipA sopABDE2 mutant | 0.3 | 1.0 | 2.3 | 3.0 | 2.7 | ||
| LB broth | 1.0 | 1.0 | 0.7 | 0.0 | 1.3 | ||
| Monocyte infiltration | Wild type | 1.5 | 3.0 | 3.3 | 3.0 | 3.5 | |
| sipA sopABDE2 mutant | 2.0 | 2.3 | 1.3 | 2.3 | 2.3 | ||
| LB broth | 1.7 | 1.0 | 1.7 | 0.7 | 2.3 | ||
| Detachment of surface epithelia | Wild type | 0.0 | 0.7 | 2.0 | 2.0 | 4.8 | |
| sipA sopABDE2 mutant | 0.3 | 0.3 | 0.0 | 0.0 | 1.0 | ||
| LB broth | 0.0 | 0.0 | 0.0 | 0.0 | 0.7 | ||
a
Each data point represents the average score from three independent experiments.
b
The histopathological changes of murine and bovine Peyer's patches were assessed in a blinded manner by two veterinary pathologists and independently scored on a scale of 0 to 5, with 5 being the highest level of histologic changes.