TABLE 2.

Primers used in this study for RT-PCR and real-time PCR

PCR type	Target	Primer sequencea	Annealing temp (°C)	No. of cycles	Product or amplicon size (bp)
RT
	b-IL-8	TGCCTAAACCCCAAGGAAAAGTG AACCCTACACCAGACCCACACAGAAC	53.5	25	205
	b-GROα	GATTCACCTCAAGAACATCCAGAGC AGAACTGCCAAACACATTCACACC	55.0	25	396
	b-GROγ	CAAAGAGGGAAAAGAGGAATCACC AAGGGCTGGCATAATGTGGG	52.0	25	335
	b-GCP-2	TTCGCCACTATGAGACTGCTATCC TCCAGACAGACTTCCCTTCCATTC	60.0	25	284
	b-MCP-1	AAACCAAACTCCAAAGCCTTGAG TTCTTGCGAGGACACTTCCACC	52.5	25	335
	b-MCP-2	ATTCTGTGTCTGCTGCTCGTGG TTCAAGGCTTCGGTGTTC	55.3	25	283
	b-MIP-1α	TCTGCCCTTGCCTGTTGTTC TCGGTGATGTATTCCTGGACCC	55.2	25	252
	b-RANTES	CCAGGAGTATTTCTACACCAGC AGCACTGAGGGTCTTTCACAGC	56.5	25	298
	m-KC	TGGGATTCACCTCAAGAAC AGTGTTGTCAGAAGCCAGCG	53.9	25	355
	m-MIP-2	CCCAGACAGAAGTCATAGCCAC AATAAGTGAACTCTCAGACAGCG	55.8	25	366
	m-GCP-2	GGCATTTCTGTTGCTGTTCACG CTTTCTTCTCTTCACTGGGGTCAG	56.3	25	343
	m-JE	GGAAAAATGGATCCACACCTTGC TCTCTTCCTCCACCACCATGCAG	58.3	25	581
	m-MCP-2	TGCTTCTTTGCCTGCTGCTC TGCTTGTAACATCTCTCTGCCTGG	56.8	25	358
	m-MIP-1α	TGACCTGGAACTGAATGC TGTGACCAACTGGAGGGATG	54.4	25	242
	m-RANTES	CATCCTCACTGCAGCCGCC CCAAGCTGGCTAGGACTAGAG	56.2	25	319
Real time
	b-IL-8	AAGTGGGTGCAGAAGGTTGTG GGAGCATGGGTTTTTCCTTTC			79
	b-GCP-2	CCAGTGTCCCCAGGAAGCT GTCCAGGAGCCTTATGGAAGTCT			103
	b-GROα	TTACTTTTTGTAGAGAAGATTGTCAGTTGTT CCAAGGGATATTTAGATCATTGTCATT			121
	b-GROγ	TTGGATGGCTGTTCCAGAAGTA GCCTTAGGAGGTGGTGATTCCT			78
	b-GAPDH	TTCTGGCAAAGTGGACATCGT GCCTTGACTGTGCCGTTGA			92
	m-KC	ACCCAAACCGAAGTCATAGCC TTCAGGGTCAAGGCAAGCC			60
	m-MIP-2	TGAGTGTGACGCCCCCA TTTTTGACCGCCCTTGAGAG			71
	m-GCP-2	ACGCTGCGCAGCATCA GCTCCGTTGCGGCTATG			59

^aSequences for bovine and murine chemokine genes were obtained from GenBank. Top row, primer 1; bottom row, primer 2.