TABLE 3.

Expression profile of chemokine genes in bovine and murine ileal Peyer's patches determined by RT-PCR

Chemokine	Loop inoculated with	Avg.a intensity of RT-PCR productsb from tissue collected at the following times (h) after inoculation of loops
		0.5	1	2	4	8
Bovine GROα	Wild type	21,659	43,862	39,525	41,398	44,374
	sipA sopABDE2 mutant	1,681	7,308	8,595	22,765	10,401
	LB broth	2,366	3,256	924	3,019	3,658
Bovine GROγ	Wild type	12,736	43,331	44,574	35,691	43,014
	sipA sopABDE2 mutant	6,159	12,780	30,285	37,517	23,707
	LB broth	3,202	5,622	3,213	4,187	9,227
Bovine IL-8	Wild type	25,839	38,272	37,428	37,213	36,497
	sipA sopABDE2 mutant	19,988	28,175	25,751	38,657	31,395
	LB broth	6,430	12,314	11,371	17,239	11,525
Bovine GCP-2	Wild type	20,906	30,238	28,735	38,998	34,338
	sipA sopABDE2 mutant	22,666	23,162	32,724	31,325	36,786
	LB broth	15,025	14,587	11,331	10,291	18,894
Bovine MCP-1	Wild type	19,865	34,807	29,242	23,868	27,201
	sipA sopABDE2 mutant	5,566	7,482	16,178	16,824	11,345
	LB broth	3,616	3,846	2,506	1,153	772
Bovine MCP-2	Wild type	38,738	49,545	44,096	44,129	52,014
	sipA sopABDE2 mutant	21,107	30,192	38,427	49,680	43,826
	LB broth	20,511	22,795	22,415	20,622	19,531
Bovine MIP-1α	Wild type	3,150	4,711	6,019	5,824	5,353
	sipA sopABDE2 mutant	7,216	4,817	3,699	6,524	5,791
	LB broth	2,067	4,096	4,977	2,330	2,483
Bovine RANTES	Wild type	41,907	42,042	42,018	34,219	39,325
	sipA sopABDE2 mutant	46,135	43,184	43,064	39,054	38,471
	LB broth	32,058	41,615	39,265	30,620	29,846
Murine KC	Wild type	47,636	52,148	50,812	52,744	53,246
	sipA sopABDE2 mutant	40,688	50,866	50,594	51,208	49,622
	LB broth	18,581	42,701	39,421	50,579	40,688
Murine GCP-2	Wild type	8,616	16,655	47,264	54,468	56,123
	sipA sopABDE2 mutant	6,878	6,359	41,984	52,237	48,917
	LB broth	4,738	5,689	15,315	45,895	39,984
Murine MIP-2	Wild type	37,232	52,471	51,698	51,403	56,766
	sipA sopABDE2 mutant	26,215	52,049	55,777	56,673	44,451
	LB broth	15,546	33,140	40,279	56,931	44,342
Murine JE	Wild type	28,370	27,814	28,592	30,706	33,599
	sipA sopABDE2 mutant	7,686	14,969	13,464	6,401	6,417
	LB broth	12,960	15,756	12,344	15,927	17,240
Murine MCP-2	Wild type	12,243	38,362	45,164	43,510	49,735
	sipA sopABDE2 mutant	1,528	25,614	43,678	48,953	35,019
	LB broth	1,732	17,727	27,713	30,071	26,441
Murine MIP-1α	Wild type	14,196	26,057	35,651	39,379	38,554
	sipA sopABDE2 mutant	18,240	13,436	27,968	29,018	18,001
	LB broth	8,580	19,396	10,920	16,760	16,433
Murine RANTES	Wild type	29,994	40,021	35,289	31,557	40,429
	sipA sopABDE2 mutant	35,293	26,349	26,708	32,241	42,878
	LB broth	32,054	19,464	32,946	40,776	38,599

^aEach data point represents the average of three independent experiments.

^bIntensity of bands was determined using the NIH image software.