TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| M. catarrhalis | ||
| O35E | Wild-type isolate | 2 |
| O35E.M | mcaP isogenic mutant of strain O35E | This study |
| O35E.ZCS | Isogenic mutant of strain O35E; but expresses McaP | 47 |
| O35E.ZCSM | mcaP isogenic mutant of strain O35E.ZCS; lacks expression of Hag, UspA1, UspA2, and McaP | This study |
| E. coli | ||
| EPI300 | Cloning strain | Epicentre |
| TOP10 | Cloning strain | Invitrogen |
| Plasmids | ||
| pCC1 | Cloning vector | Epicentre |
| pBR322 | Cloning vector | New England Biolabs |
| pUC18K1 | Source of the Kanr cassette | 38 |
| pELFOS190 | Adherent recombinant clone containing an ≈30-kb M. catarrhalis O35E.ZCS DNA insert and expressing mcaP | This study |
| pELFOS190TN6 | Adherence-negative mutant of pELFOS190 containing a transposon insertion in mcaP | This study |
| pELCCF1 | pELFOS190 digested with BamHI to remove M. catarrhalis DNA insert followed by religation of the pCC1 vector to itself | This study |
| pJTmcaP | O35E-mcaP gene cloned into pBR322 | This study |
| pJTmcaPnpkan | Kanr cassette from pUC18K1 that is introduced into the mcaP ORF carried by the plasmid pJTmcaP | This study |