TABLE 2.
Average log10 CFU/gram of tissue at day 2 and day 5 postinfection
| Dose level and tissue | Avg log10 CFU/g of tissue and log10 ratio on:
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day 2 postinfection
|
Day 5 postinfection
|
|||||||||||||||||
| wt avga,d | ΔyopB
|
ΔyopH
|
ΔyopE
|
ΔyopO
|
wt avge | ΔyopB
|
ΔyopH
|
ΔyopE
|
ΔyopO
|
|||||||||
| Avgd | Ratiob,c | Avgd | Ratio | Avgd | Ratio | Avgd | Ratio | Avgd | Ratiob,c | Avge | Ratio | Avge | Ratio | Avge | Ratio | |||
| High | ||||||||||||||||||
| Tissues | ||||||||||||||||||
| Ileum | 5.8 | 2.6 | 0.0006 | 5.5 | 0.44 | 4.8 | 0.09 | 5.5 | 0.48 | 4.9 | 2.8 | 0.01 | 4.7 | 0.79 | 4.3 | 0.26 | 4.6 | 0.62 |
| Cecum | 6.2 | 3.9 | 0.005 | 5.5 | 0.2 | 5.0 | 0.06 | 5.9 | 0.52 | 5.1 | 3.4 | 0.02 | 5.1 | 0.96 | 5.3 | 1.49 | 4.8 | 0.56 |
| Colon | 6.2 | 3.2 | 0.001 | 5.0 | 0.07 | 5.2 | 0.09 | 6.2 | 0.95 | 5.4 | 3.1 | 0.005 | 5.4 | 0.96 | 5.8 | 2.35 | 5.5 | 1.28 |
| PP | 7.0 | 3.8 | 0.0007 | 6.0 | 0.11 | 5.8 | 0.06 | 6.3 | 0.2 | 5.2 | 4.2 | 0.1 | 5.1 | 0.8 | 4.4 | 0.17 | 4.6 | 0.27 |
| CLF | 6.8 | 4.1 | 0.002 | 5.1 | 0.02 | 6.2 | 0.23 | 6.1 | 0.17 | 5.0 | 4.1 | 0.14 | 3.3 | 0.02 | 4.4 | 0.23 | 4.3 | 0.21 |
| MLN | 4.9 | 3.6 | 0.05 | 3.5 | 0.04 | 4.3 | 0.25 | 4.7 | 0.6 | 4.7 | 4.2 | 0.39 | 3.1 | 0.03 | 4.5 | 0.77 | 4.7 | 0.98 |
| Spleen | 2.9 | 2.1 | 0.17 | 2.8 | 0.95 | 2.1 | 0.17 | 2.6 | 0.52 | 4.2 | 2.3 | 0.01 | 2.6 | 0.02 | 2.3 | 0.012 | 3.4 | 0.15 |
| Low | ||||||||||||||||||
| Tissues | ||||||||||||||||||
| Ileum | 5.0 | 2.5 | 0.003 | 3.9 | 0.07 | 4.5 | 0.29 | 5.5 | 2.85 | 5.1 | 3.0 | 0.008 | 5.4 | 1.9 | 4.2 | 0.14 | 4.2 | 0.12 |
| Cecum | 5.8 | 2.6 | 0.0007 | 4.7 | 0.08 | 4.8 | 0.1 | 5.8 | 1 | 5.6 | 2.8 | 0.002 | 5.1 | 0.37 | 5.6 | 1.15 | 5.2 | 0.38 |
| Colon | 5.5 | 2.9 | 0.002 | 4.5 | 0.1 | 5.1 | 0.34 | 5.8 | 1.91 | 5.7 | 3.4 | 0.005 | 5.5 | 0.62 | 5.9 | 1.47 | 5.5 | 0.65 |
| PP | 6.6 | 2.7 | 0.0001 | 4.5 | 0.008 | 5.8 | 0.18 | 5.7 | 0.14 | 5.8 | 4.1 | 0.02 | 5.5 | 0.53 | 5.1 | 0.2 | 4.6 | 0.07 |
| CLF | 5.9 | 3.5 | 0.004 | 4.8 | 0.08 | 5.2 | 0.2 | 6.5 | 4.16 | 5.6 | 4.3 | 0.05 | 4.2 | 0.04 | 5.5 | 0.86 | 4.9 | 0.22 |
| MLN | 3.9 | 2.9 | 0.12 | 2.6 | 0.05 | 2.9 | 0.12 | 4.2 | 2.47 | 4.5 | 4.0 | 0.25 | 2.7 | 0.01 | 3.8 | 0.16 | 4.2 | 0.48 |
| Spleen | 3.2 | 2.1 | 0.08 | 2.1 | 0.08 | 2.3 | 0.11 | 2.9 | 0.45 | 4.2 | 2.7 | 0.03 | 2.2 | 0.01 | 2.0 | 0.007 | 3.0 | 0.06 |
a
avg, average of log10 CFU/gram of tissues. wt, wild type.
b
Ratio of average log10 (CFU of mutant/gram of tissue)/(average log CFU of wild type/gram of tissue).
c
Significant differences from wild type are indicated as follows: boldface, P < 0.01; underlined values, P value is between 0.01 and 0.1.
d
n = four mice pooled from two experiments.
e
n = six to eight mice pooled from three to four experiments.