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. 2003 Aug;71(8):4472–4486. doi: 10.1128/IAI.71.8.4472-4486.2003

FIG. 3.

FIG. 3.

Polymorphism in Ehssp1. (A) Schematic representation of Ehssp1-SSE58 showing the location of PCR primers and DNA probe. Numbers indicate nucleotide positions. Abbreviations: X, XmnI; H, HinfI. (B) Southern blot analysis. Genomic DNA of strain HM-1:IMSS was digested with XmnI, electrophoresed in 1% agarose gel at 4V/cm and transferred to nylon membrane. The blot was hybridized with probe 1. (C) PCR amplification of genomic DNA of strain HM-1:IMSS using ABR-F-ABR-R primer set (D) PCR amplification of genomic DNA of strain HK-9 using ABR-F-ABR-R primer set. The PCR products were electrophoresed in a 1.2% agarose gel at 4V/cm. M, molecular weight markers.