FIG. 4.

Chromosomal localization of Ehssp1. (A) Schematic representation of Ehssp1-SSE58 showing the position of DNA probes. The probes used for hybridization were derived from SSE58. Numbers indicate nucleotide positions. Abbreviations: X, XmnI; H, HinfI. (B and C) PFGE-separated chromosomes of strain HK-9 (lane 1) and HM-1:IMSS (lane 2) stained with ethidium bromide (EtBr) and hybridized with probe 2 (B) and probe 3 (C). The bands of strain HK-9 that differentially hybridized with probe 3 are shown by an asterisk. The PFGE run conditions are mentioned in Materials and Methods. Autoradiographic exposure of Southern blots was for 3 days.