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Analysis of Ehssp1 transcript. (A) Northern blot analysis. Total HM-1:IMSS RNA (30 μg) was electrophoresed in 1.2% formaldehyde agarose gel at 4 V/cm, and transferred to nylon membrane. The blot was hybridized with probe 3 (shown in Fig. 3). M, RNA molecular weight marker. RT-PCR analysis of (B) HM-1:IMSS and (C) HK9 RNA using ABR-F-ABR-R primers (Fig. 3). RT-PCR products were electrophoresed on 1.2% agarose gel at 4 V/cm. M, DNA molecular weight markers.
