FIG. 8.

Time course of reversible induction of Ehssp1 genes during heat stress. HM-1:IMSS cells growing at 36°C were transferred to 42°C. At the time points indicated, growth was stopped and RNA was isolated. To study the recovery from heat stress, cells grown at 42°C for 60 min were reverted to 36°C and RNA was isolated at different time intervals. RT-PCR was carried out using ABR-F-ABR-R primers. As controls, RT-PCR was carried out with Ehhsp101 primers (HSP-F/HSP-R, middle panel) and EhCaBP primers (CABP-F/CABP-R, bottom panel). The PCR products were electrophoresed in 1.2% agarose gel at 4 V/cm.