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A. pyogenes BBR1 binds collagen type I. Whole A. pyogenes BBR1 cells were subjected to SDS-10% (wt/vol) PAGE. Separated proteins were transferred to nitrocellulose, and Far Western blotting was performed either in the absence (−Cn) or in the presence (+Cn) of collagen type I. The positions of molecular size standards (sizes are in kilodaltons) are shown on the left. The arrow indicates the position of an approximately 120-kDa A. pyogenes protein that is able to bind collagen I.
