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. 2003 Aug;71(8):4368–4374. doi: 10.1128/IAI.71.8.4368-4374.2003

FIG. 7.

FIG. 7.

CbpA mediates adhesion of A. pyogenes strains to HeLa or 3T6 cells. (A) A. pyogenes strains were added to monolayers of HeLa (gray bars) or 3T6 (open bars) cells and allowed to adhere for 2 h at 37°C prior to washing and recovery of cell-associated bacteria. (B) HIS-CbpA, at the concentrations indicated, was preincubated with HeLa (gray bars) or 3T6 (open bars) cell monolayers for 1 h prior to addition of A. pyogenes BBR1. Adhesion is expressed as a percentage of the number of bacteria originally added to the cells. Error bars indicate one standard deviation of the mean calculated from the averages of two independent experiments performed in triplicate.