Stationary-phase survival of S. aureus strains NCTC 8325, Mu50, MRSA-252, MSSA-476, N315, COL, RF122, and MW2. Single colonies were inoculated into TSB, grown at 37°C, and aerated by shaking at 225 rpm for 8 d. At 24-h intervals, aliquots were removed, and CFU were determined in quadruplicate. Time zero on the graph represents the point at which the cultures were inoculated. The data are presented as the average and standard deviation.