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. 2003 Aug;71(8):4498–4505. doi: 10.1128/IAI.71.8.4498-4505.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 1. — PspA reactivity in pneumococcal strains. Bacterial lysates were run in sodium dodecyl sulfate-12% polyacrylamide gels and immunoblotted using monoclonal anti-PspA antibodies reactive with PspA/Rx1 (Xi126) or PspA/EF3296 (PC3.1). These two monoclonal antibodies were chosen because neither reacts with nonhomologous PspA. The samples run were EF3296 lysate (3296), TIGR4 lysate (TIGR), BR93.1 lysate (93.1), BR6.1 lysate (6.1), and WU2 lysate (WU2). The left panel shows the pattern of reactivities for the strains with Xi126 antibodies, and the right panel shows reactivities with PC3.1. EF3296, TIGR4, and BR93.1 (WU2 expressing PspA/TIGR4) lysates all showed reactivity with PC3.1 antibodies but did not react with Xi126, showing that the strains expressed only family 2 PspA. WU2 and BR6.1 (TIGR4 expressing PspA/Rx1) lysates reacted only with Xi126 antibodies, and no reactivity with PC3.1 antibodies was detected, showing that these strains expressed only the family 1 PspA. Numbers at left are molecular masses in kilodaltons.