. 2003 Aug;71(8):4506–4515. doi: 10.1128/IAI.71.8.4506-4515.2003

TABLE 1.

Features of P. chabaudi adami library clones

Library^a	No. of plasmid clones examined by restriction digestion^b	No. (%) with inserts	No. of plasmid clones examined by sequencing	Avg insert size (kb)	No. (%) with reading frames expressing peptides^c	Size range (aa) of encoded peptides	No. (%) of peptides longer than:		Avg size (aa) of encoded peptides
Library^a	No. of plasmid clones examined by restriction digestion^b	No. (%) with inserts	No. of plasmid clones examined by sequencing	Avg insert size (kb)	No. (%) with reading frames expressing peptides^c	Size range (aa) of encoded peptides	20 aa	50 aa	Avg size (aa) of encoded peptides
VR1020/30K^d	58	58 (100)	24	1.5	18 (75)	1-115	10 (42)	3 (13)	25
MCP-3/30K	32	32 (100)	22	1.3	20 (90)	3-113	8 (36)	3 (14)	32
CTLA4/30K	45	41 (93)	33	1.5	23 (70)	2-139	11 (33)	6 (18)	36

Ten plasmid pools, designated 3KA to 3KJ and each containing approximately 30,000 individual clones, were combined to create pool 30K, containing 30,000 clones.

All clones examined were from pool 3KA from each library.

Number in frame with the tissue plasminogen activator signal sequence (VR1020/30K), the MCP-3 sequence (MCP-3/30K), and the CTLA4 sequence (CTLA4/30K).

The VR1020/30K library is described in reference 31.