FIG. 7.
Investigation of growth of L. pneumophila JR32 (wild type, Mip+), JR32-2 (Mip−), JR32-2.1 (Mip+, complementation in cis), JR32-2.2 YA (Mip+; isomerase activity, 2%), JR32-2.3 DL (Mip+; isomerase activity, 6.2%), and JR32-2.4 (truncated monomer) in the guinea pig animal model. The animals were infected intratracheally with 107 bacteria. The number of recovered bacteria (CFU) was determined 48 h after infection. CFU were determined in duplicate for three independent experiments for each strain.