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. 2003 Aug;71(8):4389–4397. doi: 10.1128/IAI.71.8.4389-4397.2003

TABLE 1.

Mip expression of Legionella and recombinant E. coli strains

Strain Mip expression Relative PPIase activitya (%) Reference
L. pneumophila
    Philadelphia 1 JR32 Wild-type Mip (LpFKBP25) 100 51
    Philadelphia 1 JR32-2 Mip negative 0.0 51
    Philadelphia 1 JR32-2.1 Mutant complemented in cis with wild-type Mip (LpFKBP25) 100 51
    Philadelphia 1 JR32-2.2 Site-specifically altered Mip [FKBP25 (Y185A)] 2.0 51
    Philadelphia 1 JR32-2.3 Site-specifically altered Mip [FKBP25 (D142L)] 6.2 51
    Philadelphia 1 JR32-2.4 N-terminally truncated monomeric Mip [LpFKBP25(−20-3,80-213)] 100 This study
E. coli DH5α/pJMFe991 N-terminally truncated monomeric Mip [Mip(77-213)] 100 This study
a

PPIase activity was determined relative to the wild-type Mip protein by the standard protease-coupled assay as described previously (11, 35) with Suc-Ala-Phe-Pro-Phe-4NA as substrate.