TABLE 2.

Kinetic constants for refolding of RCM-T₁ by different PPIases

PPIase	kcat (S−1)	Km (nM)	kcat/Km (s−1 M−1)a	Reference
Mip	0.095	75	1.2 × 106	This study
Mip(77-213)	NDb	ND	0.028 × 106	This study
E. coli trigger factor	1.3	700	1.8 × 106	45
E. coli FkpA	0.5	300	1.6 × 106	37

^aDifferences from the values in Fig. 3 arise because two different methods were used. The values for Mip, E. coli trigger factor, and E. coli FkpA were calculated from the single constants k_cat and K_m whereas the values for k_cat/K_m in Fig. 3 were determined directly.

^bND, not determined.