FIG. 5.

Western blot analysis of the reactivity of anti-AMA49-L1 (A), anti-AMA49-C1 (B), and anti-AMA49-L2 (C) antisera with P. falciparum K1 blood stage lysates as the antigen. Sera yielded a double band pattern (indicated by arrows) characteristic of full-length AMA-1 and its major processing product (83 and 66 kDa, respectively). Each lane represents serum from one individual mouse. No staining was observed with the corresponding preimmune serum samples. All serum samples were used at a dilution of 1:100.