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TLC stained with anisaldehyde (A) and immunostained chromatogram obtained by overlay with A. pleuropneumoniae serotype 1 cells (B). Lanes contain different concentrations of GlcCer (I), LacCer (II), GgO3 (III), or GgO4 (IV) as follows: lane 1, 10 μg; lane 2, 5 μg; lane 3, 2.5 μg; lane 4, 1.25 μg; lane 5, 0.63 μg; lane 6, 0.31 μg; lane 7, 0.16 μg; lane 8, 0.08 μg; lane 9, 0.04 μg; lane 10, 0.02 μg; lane 11, 0.01 μg.
