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Identification of the recombinant plasmid by digestion with BamHI plus HindIII and PCR. Lane M, λ DNA/EcoT14I standard marker; lane 1, recombinant plasmid pQE30/56 digested with BamHI plus HindIII; lane 2, pQE30 digested with BamHI plus HindIII; lane 3, PCR product of recombinant plasmid pQE30/56.
