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. 2003 Aug;71(8):4772–4779. doi: 10.1128/IAI.71.8.4772-4779.2003

FIG. 7.

FIG. 7.

Immunoblot analysis of the recombinant Sxh56 protein produced by the pQE30/sxh56 clone. Crude cell extracts were obtained from E. coli cells containing recombinant pQE30/sxh56 plasmids, separated by SDS-10% PAGE, transferred to nitrocellulose membranes, and reacted with anti-O. tsutsugamushi Sxh951 antibodies (see Materials and Methods). Molecular masses are indicated on the left. Lane M, protein molecular weight marker; lane 1, E. coli M15 harboring pQE30/sxh56 induced by IPTG; lane 2, E. coli M15 harboring pQE30 induced by IPTG; lane 3, E. coli M15 induced by IPTG.