TABLE 2.
In vitro proliferation in response to recombinant Sxh56 proteins of T cells from mice immunized with recombinant Sxh56 proteina
| Group | Proliferation (OD570 − OD630) with the following concn (μg/ml) of recombinant Sxh56 protein:
|
||||
|---|---|---|---|---|---|
| 10 | 3 | 1 | 0.3 | 0 | |
| PBS control | 0.423 | 0.437 | 0.476 | 0.420 | 0.407 |
| Recombinant Sxh56 | 0.453 | 0.565 | 0.643 | 0.604 | 0.414 |
a
BALB/c mice (four per group) were immunized subcutaneously with PBS or recombinant Sxh56 protein. Eight days after the first booster, spleens of four mice per group were harvested and T-cell cultures were stimulated in vitro for 3 days with medium or with various concentrations of purified recombinant Sxh56 proteins. Significant differences were found between the groups of mice immunized with Sxh56 or PBS (P < 0.05 [0.045] by t test).