FIG. 1.

Proliferative response of lymph node cells to Rpf-like proteins. Mice were immunized with individual proteins of the Rpf family as described in Materials and Methods (A) or left unimmunized (B). Two weeks after the last immunization, cells were isolated from auxiliary and inguinal lymph nodes of three mice in each group, mixed, and stimulated in vitro with 10 μg of an individual antigen (Ag) (shaded bars) per ml or left unstimulated (open bars). All cultures were performed in triplicate. Cultures were pulsed with [³H]thymidine (0.5 μCi/ml) for the last 18 of 72 h of incubation. Results of one out of two similar experiments are expressed as mean counts per minute ± SDs from triplicate cultures.