FIG. 3.

Cytokine production by lymph node cells from mice immunized with individual Rpf-like proteins. The mixture of cells from three mice was either additionally stimulated in vitro with a corresponding antigen (Ag) (shaded bars) or left unstimulated (open bars), and the amounts of IFN-γ (A), IL-10 (B), and IL-12 (C) in supernatants were assessed by ELISA as described in Materials and Methods. The amounts of IL-4 and IL-5 were always below the sensitivities of the corresponding assay. Results (in picograms per milliliter) are expressed as the means of results from three similar experiments ± standard errors of the means.