TABLE 1.
Parasite isolate | Origin | Selected for CSA binding | Recognized by female but not male antibodies | % of iRBC binding IgM |
---|---|---|---|---|
SD2H3 | Sudan | No | No | 1 |
SD2H3CSA | Yes | Yes | 74 | |
SD202 | Sudan | No | No | 4 |
SD202CSA | Yes | Yes | 94 | |
Busua | Ghana | No | No | 9 |
BusuaCSA | Yes | Yes | 91 | |
Gb337 | Gabon | No | No | 2 |
Gb337CSA | Yes | Yes | 79 | |
HB3 | Honduras | No | No | 4 |
HB3CSA | Yes | No | 47 | |
FCR3 | Gambia | No | No | 4 |
FCR3CSA | Yes | Yes | 88 |
Results are given as the percentage of IgM-positive iRBC observed in a representative experiment on each line. All lines were tested at least three times, some as many as 10 times. Consistent results were obtained in all experiments. There was variation in the time at which each culture reached sufficient density to allow testing and also in the rate at which different lines lost their selected adhesion property (27). This precluded averaging results, because the timing of each test postselection will not be strictly comparable.