TABLE 2.
MAb | Specificity | Source or reference | % IgM positivea |
---|---|---|---|
Human IgM-positive control | Not applicable | Human serum | 78 |
Negative control (goat anti-mouse IgM secondary antibody) | Not applicable | Molecular Probes | 0 |
MAb 2040 | Heparan sulfate | Chemicon | 80 |
SM phage antibody | CD36 | Sigma | 74 |
MAb CS-56 | Chondroitin sulfate | Sigma | 77 |
MAb MOPC-104E | IgM isotype control | Sigma | 78 |
MAb TCN-1 | Toxocara canis | 24 | 60 |
MAb TCN-4 | Toxocara canis | 24 | 82 |
MAb TCN-8 | Toxocara canis | 24 | 82 |
Data from a representative experiment are shown. Three further experiments gave consistent results. The unselected parental line Gb337 was also tested, and all MAbs showed less than 2% positive infected cells by IFA.