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Expression of IFN-γ, IL-10, and IL-5 in the spleens (A) and lungs (B) of BALB/c mice. The mice were immunized i.n. with type 3 pneumococcal conjugate vaccine plus IL-12 or PBS vehicle. Control mice received PBS vehicle only. The mice were sacrificed 48 h after treatment, total RNA was isolated from individual spleen and lungs, and expression of IFN-γ, IL-10, and IL-5 was analyzed by RT-PCR. HPRT was used as a housekeeping control.
