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. 2006 Nov 9;7:40. doi: 10.1186/1471-2199-7-40

Figure 2.

Figure 2

Targeted silencing by Esc4 is SIR-dependent. Targeted silencing by GBD-Esc4 (1–1070) was assessed in hmr::URA3 silencing reporter strains containing different HMR-E elements replaced by a Gal4 DNA binding site (G), similar to the situation shown in Figure 1B. A TRP1-marked plasmid expressing GBD alone served as a negative control and targeted silencing by the potent targeted silencing factor GBD-Esc1(1124–1658) served as a positive control [17]. Targeted silencing by GBD-Esc4 in strains with wild-type SIR genes (SIR+) or sir2Δ, sir3Δ, or sir4Δ mutations was examined.